5 parameter polynomial curve Search Results


prism 9.5.1 standard curve interpolation (four parameter) tool  (GraphPad Software Inc)
90
GraphPad Software Inc prism 9.5.1 standard curve interpolation (four parameter) tool
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Prism 9.5.1 Standard Curve Interpolation (Four Parameter) Tool, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism 9.5.1 standard curve interpolation (four parameter) tool/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism 9.5.1 standard curve interpolation (four parameter) tool - by Bioz Stars, 2026-06
90/100 stars
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dose–response curve fit with a 5-parameter non-linear function using graphpad prism 8  (GraphPad Software Inc)
90
GraphPad Software Inc dose–response curve fit with a 5-parameter non-linear function using graphpad prism 8
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Dose–Response Curve Fit With A 5 Parameter Non Linear Function Using Graphpad Prism 8, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dose–response curve fit with a 5-parameter non-linear function using graphpad prism 8/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
dose–response curve fit with a 5-parameter non-linear function using graphpad prism 8 - by Bioz Stars, 2026-06
90/100 stars
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non-linear regression analysis log dose response 4-parameter fit curves graphpad prism 5.01  (GraphPad Software Inc)
90
GraphPad Software Inc non-linear regression analysis log dose response 4-parameter fit curves graphpad prism 5.01
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Non Linear Regression Analysis Log Dose Response 4 Parameter Fit Curves Graphpad Prism 5.01, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/non-linear regression analysis log dose response 4-parameter fit curves graphpad prism 5.01/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
non-linear regression analysis log dose response 4-parameter fit curves graphpad prism 5.01 - by Bioz Stars, 2026-06
90/100 stars
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5-parameter curve fitting (labkey nab tool)  (LabKey Corporation)
90
LabKey Corporation 5-parameter curve fitting (labkey nab tool)
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
5 Parameter Curve Fitting (Labkey Nab Tool), supplied by LabKey Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5-parameter curve fitting (labkey nab tool)/product/LabKey Corporation
Average 90 stars, based on 1 article reviews
5-parameter curve fitting (labkey nab tool) - by Bioz Stars, 2026-06
90/100 stars
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four parameter sigmoidal dose-response curve graphpad prism software version 5.04  (GraphPad Software Inc)
90
GraphPad Software Inc four parameter sigmoidal dose-response curve graphpad prism software version 5.04
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Four Parameter Sigmoidal Dose Response Curve Graphpad Prism Software Version 5.04, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/four parameter sigmoidal dose-response curve graphpad prism software version 5.04/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
four parameter sigmoidal dose-response curve graphpad prism software version 5.04 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
three-parameter curve fit function in graphpad prism v. 5.01  (GraphPad Software Inc)
90
GraphPad Software Inc three-parameter curve fit function in graphpad prism v. 5.01
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Three Parameter Curve Fit Function In Graphpad Prism V. 5.01, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/three-parameter curve fit function in graphpad prism v. 5.01/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
three-parameter curve fit function in graphpad prism v. 5.01 - by Bioz Stars, 2026-06
90/100 stars
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5-parameter logistic (5pl) curve  (GraphPad Software Inc)
90
GraphPad Software Inc 5-parameter logistic (5pl) curve
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
5 Parameter Logistic (5pl) Curve, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5-parameter logistic (5pl) curve/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
5-parameter logistic (5pl) curve - by Bioz Stars, 2026-06
90/100 stars
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r-strip1 polyexponential curve stripping/parameter estimation software, version 5.0  (MicroMath Inc)
90
MicroMath Inc r-strip1 polyexponential curve stripping/parameter estimation software, version 5.0
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
R Strip1 Polyexponential Curve Stripping/Parameter Estimation Software, Version 5.0, supplied by MicroMath Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r-strip1 polyexponential curve stripping/parameter estimation software, version 5.0/product/MicroMath Inc
Average 90 stars, based on 1 article reviews
r-strip1 polyexponential curve stripping/parameter estimation software, version 5.0 - by Bioz Stars, 2026-06
90/100 stars
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log (inhibitor) versus response curves (three parameters) with a hill slope of −1.0 graphpad prism 5  (GraphPad Software Inc)
90
GraphPad Software Inc log (inhibitor) versus response curves (three parameters) with a hill slope of −1.0 graphpad prism 5
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Log (Inhibitor) Versus Response Curves (Three Parameters) With A Hill Slope Of −1.0 Graphpad Prism 5, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/log (inhibitor) versus response curves (three parameters) with a hill slope of −1.0 graphpad prism 5/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
log (inhibitor) versus response curves (three parameters) with a hill slope of −1.0 graphpad prism 5 - by Bioz Stars, 2026-06
90/100 stars
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computer program based on the five parameters polynomial equation 'generic curve  (GraphPad Software Inc)
90
GraphPad Software Inc computer program based on the five parameters polynomial equation 'generic curve
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Computer Program Based On The Five Parameters Polynomial Equation 'Generic Curve, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/computer program based on the five parameters polynomial equation 'generic curve/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
computer program based on the five parameters polynomial equation 'generic curve - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
four-parameter sigmoidal dose–response curves graphpad prism 5.04  (GraphPad Software Inc)
90
GraphPad Software Inc four-parameter sigmoidal dose–response curves graphpad prism 5.04
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Four Parameter Sigmoidal Dose–Response Curves Graphpad Prism 5.04, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/four-parameter sigmoidal dose–response curves graphpad prism 5.04/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
four-parameter sigmoidal dose–response curves graphpad prism 5.04 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
four parameter logistic curve fitting program deltasoft jv 5  (DeltaSoft Inc)
90
DeltaSoft Inc four parameter logistic curve fitting program deltasoft jv 5
Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.
Four Parameter Logistic Curve Fitting Program Deltasoft Jv 5, supplied by DeltaSoft Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/four parameter logistic curve fitting program deltasoft jv 5/product/DeltaSoft Inc
Average 90 stars, based on 1 article reviews
four parameter logistic curve fitting program deltasoft jv 5 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.

Journal: Scientific Reports

Article Title: Engineering a GPCR-based yeast biosensor for a highly sensitive melatonin detection from fermented beverages

doi: 10.1038/s41598-024-68633-y

Figure Lengend Snippet: Original biosensor features. ( A ) GPCR integration cassette, this strain has MTNR1A integrated in LEU2 locus. ( B ) Reporter integration cassette bearing a synthetic transcription factor (LexA-PRD), a synthetic promoter driving the expression of the reporter gene sfGFP and a Gα (GPA1) overexpression. This cassette is integrated in URA3 locus. ( C ) Curve fitting of yWS1544 strain derived from Shaw et al. . Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.

Article Snippet: Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool.

Techniques: Expressing, Over Expression, Derivative Assay, Fluorescence

Modifications on original strain affecting melatonin sensing properties. A comparison of the dose–response between original biosensor strain and after increasing MTNR1A receptor number (yRB1002) ( A ), after increasing MTNR1A and adding one extra copy of the reporter cassette (yRB1012) ( B ) and after increasing MTNR1A and integrating reporter cassette multiple times (yRB1022) ( C ). Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.

Journal: Scientific Reports

Article Title: Engineering a GPCR-based yeast biosensor for a highly sensitive melatonin detection from fermented beverages

doi: 10.1038/s41598-024-68633-y

Figure Lengend Snippet: Modifications on original strain affecting melatonin sensing properties. A comparison of the dose–response between original biosensor strain and after increasing MTNR1A receptor number (yRB1002) ( A ), after increasing MTNR1A and adding one extra copy of the reporter cassette (yRB1012) ( B ) and after increasing MTNR1A and integrating reporter cassette multiple times (yRB1022) ( C ). Measurements are normalized sfGFP fluorescence levels and shown as mean ± SD of triplicates. Curve fitting and parameters were obtained using GraphPad Prism 9.5.1 variable slope (four parameter) nonlinear regression fit.

Article Snippet: Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool.

Techniques: Comparison, Fluorescence

Screening of 101 yeast strains. Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool. Melatonin concentration values are shown as mean ± SD of triplicates. 17 of the 101 strains assayed were excluded from interpolation due to lack of enough signal.

Journal: Scientific Reports

Article Title: Engineering a GPCR-based yeast biosensor for a highly sensitive melatonin detection from fermented beverages

doi: 10.1038/s41598-024-68633-y

Figure Lengend Snippet: Screening of 101 yeast strains. Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool. Melatonin concentration values are shown as mean ± SD of triplicates. 17 of the 101 strains assayed were excluded from interpolation due to lack of enough signal.

Article Snippet: Yeast biosensor strain yRB1012 was employed in this assay. sfGFP fluorescence for each sample was interpolated in the fitted calibration curve for melatonin using GraphPad Prism 9.5.1 standard curve interpolation (four parameter) tool.

Techniques: Fluorescence, Concentration Assay